Plant Tissue Culture: Totipotency to Transgenic

Fundamental and recent trends in research activities and application of latest techniques have been taken in to account. The book has been divided in two parts; Part-I comprises Fundamental Techniques and Part- II comprises Molecular Techniques and Applications. The former describes basics of plant tissue culture through different chapters, such as Laboratory organization, Sterilization Technique, Media Preparation, Embryo Culture, Somatic Embryogenesis, Haploid Culture, Endosperm Culture, Protoplast Culture and Somatic Hybridization, In vitro Pollination and Fertilization, Somaclonal Variation, Micropropagation and Acclimatization. The Part-II discusses Genetic Transformation, Hairy Root Culture, Enhancement of Secondary Metabolites, Role of Metal as Elicitors, Molecular Markers, Genetic Fidelity, Microarray, Bioinformatics and Nanotechnology. A comprehensive Glossary, Subject Index and the exhaustive references at the end of chapters have made the book more useful and reader-friendly.

1. Plant Tissue Culture: An Overview H. P. Sharma

2. Tissue Culture Laboratory Organization V. K. Shahi and Shailesh Kumar

3. Plant Tissue Culture Media Dr. J. V. V. Dogra

4. Sterilization Techniques in Plant Tissue Culture Amarendra Narayan Misra and Meena Misra

5. Somatic Embryogenesis and its Application G. R. Rout and C. Pradhan

6. In Vitro Organogenesis: Induction, Regulation and Applications G. S. Shekhawat and Sonali Jana

7. Synthetic Seed Santi Lata Sahoo, Jyoti Ranjan Rout and Satyajit Kanungo

8. Haploid Plant: Production, Prospective and Application Kambaska Kumar Behera, Biswanath Bhunia and H. P. Sharma

9. Endosperm Tissue Culture: Technique and its Potential Application in Plant Improvement Nishi Kumari

10. In Vitro Pollination and Fertilization- A Recent Update Veena Agrawal and Kuldeep Sharma

11. Embryo Culture: A Technical Approach to Overcome Hybrid Inviability Mohammad Anis, Shahnaz Parveen, Anushi. A. Jahan and Ibrahim M. Aref

12. Protoplast Culture, Fusion and Somatic Hybridization Y.K. Bansal and Shikha Bansal

13. Somaclonal Variation Kavindra Nath Tiwari, Shikha Suman, Jayanti Singh and Pratima Kapur

14. Agrobacterium tumefaciens Mediated Genetic Transformation Prof. A. Sadanandam Dr. A. Venkateshwar Rao and Dr. T. Christopher

15. Transgenic Banana: Challenges and Opportunities V. A. Bapat and T.R Ganapathi, U. K. S. Shekhawat

16. Plastid Transformation in Higher Plants Sadanandam, K. Venugopal Rao, P. Venkataiah, A. Venkateshwar Rao, and T.Christopher

17. Plant Propagation through In Vitro Production of Storage Organs Dr. C. K. John

18. Micropropagation K. Jaganmohan Reddy and M. Venkateshwarlu

19. Secondary Plant Products: Therapeutic Potential and Production through Plant Tissue Culture Kishan G. Ramawat and Shaily Goyal, Jaya Arora

20. Transgenic Hairy Roots: Development and Applications Sheela Chandra, M. M. Sharma

21. Role of Plant Tissue Culture in Conservation of Plant Genetic Resources K. Sharma, Kavita Arora, Meena Sharma and Madhu Jain

22. Role of Metal Ions as Potential Abiotic Elicitors of Secondary Metabolites in Plant Cell, Tissue and Organ Culture Techniques Bhumi Nath Tripathi and Vijeta Singh

23. Biogenic Synthesis of Metal Nanoparticles Using Plants Dr. Rajesh Thakur

24. Bioinformatics in Relation to Plant Biotechnology Gaurab Gangopadhyay and Sangita Basu Gangopadhyay

25. Applications of Molecular Markers in Plant Tissue Culture K. Misra and Suman Kumaria

26. Assessment of Genetic Fidelity of the Tissue Culture Clones Gaurab Gangopadhyay and Kalyan K Mukherjee

27. Role of Microarray in Plant Tissue Culture Amla Batra, Abhijeet Singh, Madan Mohan Sharma and Deevan Julfikar Ali

28. Acclimatization of Tissue Cultured Plantlets Madhuparna Banerjee

29. Plant Cell Tissue Culture and its Industrial Application H. P. Sharma

Glossary

Abbreviations